Green’s Dictionary of Slang

chamber lye n.

also chamber lee
[play on SE chamber(pot) + lye/lie; note late 19C–early 20C Southern US black use chamber lye, urine sprinkled around a garden to keep wandering deer away]

urine standing in a chamberpot.

B. Googe Heresbach’s Husb. III. (1586) 135b: Take Chamberly, and Salte, and seeth them to gether, and washe the places where the skinne is cut of.
[UK]Shakespeare Henry IV Pt 1 II i: Your chamber-lie breeds fleas like a loach.
[UK]C. Cotton Virgil Travestie (1765) Bk I 51: For know that she / Still wash’d her Hands in Chamber-lee.
[Ire]‘Teague’ Teagueland Jests I 98: She threw a full Pot of Chamber-lee upon him.
[UK]N. Ward London Spy III 65: We peep’d into another Room, which smelt as strong of Chamber-Lie as a Bottle of Sal Ammoniac.
[UK]Grose Classical Dict. of the Vulgar Tongue.
[UK]Lex. Balatronicum.
[UK]Egan Grose’s Classical Dict. of the Vulgar Tongue.
[US]Matsell Vocabulum.
[US] Confederate Army notice, Montgomery (AL) in Randolph & Legman Ozark Folksongs and Folklore (1992) II 659: The ladies of Montgomery are respectfully requested to save all the chamber lye that accumulates on their premises [etc.].
[US]Monteleone Criminal Sl. (rev. edn).
S. Harvey Bound for Shady Grove 51: Fill up the gallon glass jug with chamber-lye from your bed pot.