Green’s Dictionary of Slang

chamber of commerce n.

[SE chamber, a lavatory + pun on business n. (2)]

(US black/campus) a lavatory.

[US]E.H. Babbitt ‘College Words and Phrases’ in DN II:i 26: chamber of commerce, n. Water-closet.
[US] ‘Jiver’s Bible’ in D. Burley Orig. Hbk of Harlem Jive.
[US]Wentworth & Flexner DAS.